Western Blot of A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using RM214 at 0.5 ug/mL, showed endogenous Histone H2AX in A375, HEK293, HeLa and SK-MEL-2 cells.
Immunocytochemistry of HeLa cells, using Histone H2AX Rabbit mAb RM214 (red). Actin filaments have been labeled with fluorescein phalloidin (green).